Vergleich von Polyethylenterephthalat-hydrolysierenden Cutinase-Varianten aus Thermobifida fusca

Characterization of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins and their potential application in bioscouring.

Cutinase from Thermobifida fusca is thermally stable and has potential application in the bioscouring of cotton in the textile industry. In the present study, the carbohydrate-binding modules (CBMs) from T. fusca cellulase Cel6A (CBM(Cel6A)) and Cellulomonas fimi cellulase CenA (CBM(CenA)) were fused, separately, to the carboxyl terminus of T. fusca cutinase. Both fusion enzymes, cutinase-CBM(C...

Tabellarischer Vergleich von Prozessmodellen für modellbasiertes Testen aus Managementsicht

Testmodelle spielen also eine zentrale Rolle beim MBT und müssen zuerst erstellt werden. Das bedarf oft zusätzlicher Kosten. Ein Testmanager, der MBT einsetzen möchte, muss nicht nur die Erstellung, sondern auch die Verwaltung von Testmodellen in der Kostenplanung berücksichtigen. Die Schulung des Testpersonals und Beschaffung von Werkzeugen stellen weitere Kostenpunkte dar. Die Zusatzkosten, d...

Varianten von Hensels Lemma

Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

BACKGROUND Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory system in Escherichia coli BL21(DE3), and it was found that parts of the expressed protein was accumulated in the periplasmic space. Due to the fact...

Kinetic mechanism of phenylacetone monooxygenase from Thermobifida fusca.

Phenylacetone monooxygenase (PAMO) from Thermobifida fusca is a FAD-containing Baeyer-Villiger monooxygenase (BVMO). To elucidate the mechanism of conversion of phenylacetone by PAMO, we have performed a detailed steady-state and pre-steady-state kinetic analysis. In the catalytic cycle ( k cat = 3.1 s (-1)), rapid binding of NADPH ( K d = 0.7 microM) is followed by a transfer of the 4( R)-hydr...